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11 changes: 11 additions & 0 deletions CHANGELOG.md
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The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).


## [3.2.3] - 2024-XX-XX

### Enhancements

- ...

### Fixes

- Fixed output documentation where sections 5 and 6 of output docs were hidden

## [3.2.2] - 2024-02-01

### Enhancements
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4 changes: 1 addition & 3 deletions docs/output.md
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Expand Up @@ -263,7 +263,7 @@ High levels of duplication are not necessarily a problem as long as they are con
- `.la_dedup.bam`: Coordinate sorted BAM file after Linear Amplification duplicate removal. This is the final post-processed BAM file and so will be saved by default in the results directory.
- `.la_dedup.bam.bai`: BAI index file for coordinate sorted BAM file after Linear Amplification duplicate removal. This is the final post-processed BAM index file and so will be saved by default in the results directory.
- `.la_dedup_metrics.txt`: Metrics file from custom deduplication based on read 1 5' start location.

</details>
In assays where linear amplification is used, the resulting library may contain reads that share the same start site but have a unique 3' end due to random cut and tagmentation with Tn5-ME-B. In this case, these duplicates should be removed by filtering all read 1's based on their 5' start site and keeping the read aligning with the highest mapping quality.

## 5. <a name='Fragment-basedQC'></a>Fragment-based QC
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### 6.5. <a name='ConsensusPeaks'></a>Consensus Peaks

</details>

The merge function from [BEDtools](https://github.com/arq5x/bedtools2) is used to merge replicate peaks of the same experimental group to create a consensus peak set. This can then optionally be filtered for consensus peaks contributed to be a threshold number of replicates using `--replicate_threshold`.

## 7. <a name='Peak-basedQC'></a>Peak-based QC
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