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Weaning age alters intestinal stabilization of intraepithelial T lymphocytes

Scripts found here are associated with multidimensional analysis of flow cytometry data from the below work:

Weaning age alters intestinal stabilization of intraepithelial T lymphocytes and mucosal microbiota in pigs by Jayne E. Wiarda, Hannah R. Watkins, Melissa S. Monson, Christopher L. Anderson, Crystal L. Loving

Peer-reviewed publication: TBD this work has not been accepted for peer-reviewed publication and is still subject to alterations

Repository organization

File Name Description
FCS_CD3Epos Directory containing FCS files of unprocessed data imported for analysis
FCS_processed Directory containing FCS files of fully processed data used for analysis
FC_DataPrep_HighDimDA_20230914.R R script detailing data analysis
FS24meta.csv File containing meta data information of samples used for analysis
README.md The repository README file... you're reading this now :)

Additional materials

Additional data associated with this project can also be found as detailed below:

  • Raw sequencing data can be found in the Sequence Read Archive (SRA) under BioProject ID ____ (the sequencing data will be released upon publication)
  • Analysis of 16S rRNA sequencing data: github_

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Code and materials to support work analyzing flow cytometry data of T-IELs taken from standard- or late-weaned pigs by Wiarda et al.

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