diff --git a/packages/google-cloud-spanner/noxfile.py b/packages/google-cloud-spanner/noxfile.py
index 39c7c32036b4..dd6db62994e8 100644
--- a/packages/google-cloud-spanner/noxfile.py
+++ b/packages/google-cloud-spanner/noxfile.py
@@ -71,7 +71,6 @@
 CURRENT_DIRECTORY = pathlib.Path(__file__).parent.absolute()
 
 nox.options.sessions = [
-    "unit-3.9",
     "unit-3.10",
     "unit-3.11",
     "unit-3.12",