When I follow instructions for building reference with Bowtie2 then when calculating the expression I get the error:
No genome information provided, so genome bam file cannot be generated!
But when using the pre-built reference it works fine. I confirmed all the files are there after building reference.
Any ideas?
[EDIT] It may be worth to mention this only occurs when building using the 2nd option: building references from a set of transcripts.
When I follow instructions for building reference with Bowtie2 then when calculating the expression I get the error:
No genome information provided, so genome bam file cannot be generated!But when using the pre-built reference it works fine. I confirmed all the files are there after building reference.
Any ideas?
[EDIT] It may be worth to mention this only occurs when building using the 2nd option: building references from a set of transcripts.